By Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung
This guide presents technical protocols for musculoskeletal study on a translational foundation, i.e. a disease-orientated strategy. It bargains counsel on a number of laboratory innovations, together with mobile tradition and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal versions, CT- and MRI-based densitometry and microarchitectural research, biomechanics and sensible research of orthopedic kinesiology, and so forth. The content material is straightforward and easy, with illustrations and step by step strategies as a simple experimental reference for team of workers in simple and medical musculoskeletal study and schooling. This booklet will supply a special multidisciplinary platform for numerous professions -- not just orthopedics, but in addition biomedical engineering and biomaterial sciences -- concerning either uncomplicated and scientific drugs.
Contents: cellphone tradition and Molecular Biology; Histology and Histomorphometry; Microscopy and Bioimaging; Laboratory Animal types; X-Ray- and MRI-based Densitometry; Biomechanics and movement research
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G. formamide) at specific temperatures. These solutions can be purchased from reliable suppliers who guarantee that they will work in specific conditions. • • • • • g Place the slide in a hybridization cassette. 0 µL of ddH2O to the slot in the cassette to prevent drying of the sample. Mix the Cy3-labeled reference probe with the Cy5-labeled test probe in a 1:1 ratio. Boil the mixed probes for 2 minutes and spin briefly at 13 000 g. 7 µL/cm2 of the mixed probe onto the microarray, place a cover slip onto the slide using forceps,g close the hybridization cassette containing the microarray, and submerge the hybridization cassette in a water bath set at 62°C.
A Practical Manual for Musculoskeletal Research by Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung
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