By Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung

ISBN-10: 9812706100

ISBN-13: 9789812706102

This guide presents technical protocols for musculoskeletal study on a translational foundation, i.e. a disease-orientated strategy. It bargains counsel on a number of laboratory innovations, together with mobile tradition and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal versions, CT- and MRI-based densitometry and microarchitectural research, biomechanics and sensible research of orthopedic kinesiology, and so forth. The content material is straightforward and easy, with illustrations and step by step strategies as a simple experimental reference for team of workers in simple and medical musculoskeletal study and schooling. This booklet will supply a special multidisciplinary platform for numerous professions -- not just orthopedics, but in addition biomedical engineering and biomaterial sciences -- concerning either uncomplicated and scientific drugs.

Contents: cellphone tradition and Molecular Biology; Histology and Histomorphometry; Microscopy and Bioimaging; Laboratory Animal types; X-Ray- and MRI-based Densitometry; Biomechanics and movement research

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Therefore, data acquisition and analysis must be automated. The software for initial image processing is normally provided with the scanner; this allows the boundaries of individual spots to be determined and the total signal intensity to be measured over the whole spot (signal volume). The signal intensity should be corrected for background, and control measures should be included to measure nonspecific hybridization and variable hybridization across arrays. The aim of data mining is to convert the hybridization signals into numbers which can be used to build a gene expression matrix.

Xiao Y, Fu H, Prasadam I et al. Gene expression profiling of bone marrow stromal cells from juvenile, adult, aged and osteoporotic rats: with an emphasis on osteoporosis. Bone 40:700–715, 2007. Zheng PZ, Wang KK, Zhang QY et al. Systems analysis of transcriptome and proteome in retinoic acid/arsenic trioxide combination-induced cell differentiation/apoptosis of acute promyelocytic leukemia. Proc Natl Acad Sci USA 102:7653–7658, 2005.

G. formamide) at specific temperatures. These solutions can be purchased from reliable suppliers who guarantee that they will work in specific conditions. • • • • • g Place the slide in a hybridization cassette. 0 µL of ddH2O to the slot in the cassette to prevent drying of the sample. Mix the Cy3-labeled reference probe with the Cy5-labeled test probe in a 1:1 ratio. Boil the mixed probes for 2 minutes and spin briefly at 13 000 g. 7 µL/cm2 of the mixed probe onto the microarray, place a cover slip onto the slide using forceps,g close the hybridization cassette containing the microarray, and submerge the hybridization cassette in a water bath set at 62°C.

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A Practical Manual for Musculoskeletal Research by Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung


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